The Nebraska Amish are perhaps the most conservative group of Old Order Amish, descendants of the Anabaptists and Mennonites.

The present Nebraska Amish are found in Mifflin, Union, and Eastern Penns Valley.  The Amish came into this region of Pennsylvania as early as 1791. Around 1880, Bishop Yost H. Yoder led nine families from Pennsylvania to south-central Nebraska, founding an Old Order settlement that would last until 1904, three years after Bishop Yoder's death.  Yoder went back to the Kishacoquillas Valley in Pennsylvania in 1881 to assist a conservative Amish group.  Yoder was living in Nebraska, and the group was nicknamed the Nebraska Amish by others.

Like other Old Order Amish, the Nebraska Amish do not use motorized equipment or indoor plumbing, and wear very conservative clothing. Differences include the fact that the men do not wear suspenders and the women do not wear bonnets.  Other differences include the fact that they do not place screens on their doors or windows, men only wear white shirts, curtains are not used in homes, buggy tops must be white, men's hair must be shoulder length, no lawn mowers are allowed and houses must not have projecting roofs.

A group called the Zook faction broke away from the Yoders in 1933, and constitute a separate "district", holding their own worship services and having their own bishops. Though differences exist, they are unnoticeable to outsiders. There are less than 1,000 member of the Nebraska Amish in the area around Centre and Mifflin Counties.